E101(iv) – Riboflavin from Ashbya gossypii

Additive E101(iv)

Other names for the additive (synonyms)

E 101(iv), E-101(iv), E 101 (iv)

General Information

The food additive E101(iv) is a subtype of the food additive E101 and is riboflavin produced through fermentation using the fungus Ashbya gossypii.

Chemically, it is the same riboflavin with the formula C₁₇H₂₀N₄O₆, but the primary distinction lies in its method of production.

Production is carried out entirely via biotechnological fermentation: selected strains of Ashbya gossypii are cultivated in a nutrient medium where they synthesize riboflavin in significant quantities. After fermentation, the product is purified and dried. This approach is considered environmentally safe and aligns with the concept of “biosynthesis.”

In terms of properties, E101(iv) is identical to naturally derived riboflavin: it has an intense yellow color, limited solubility in water, and is sensitive to light. It tolerates heat processing well and remains stable in most food matrices.

In the food industry, the E101(iv) additive is used as a natural colorant and vitamin supplement in “clean label” products, organic foods, and functional foods. Its use allows manufacturers to emphasize the biotechnological and natural origin of the ingredients.

For more detailed information on the properties of riboflavins, their effects on the human body, and references to scientific studies, see the material on the food additive E101.