Other names for the additive (synonyms)
General Information
The food additive E101(iii) is a subtype of the food additive E101 and is riboflavin produced by fermentation using Bacillus subtilis bacteria.
Chemically, it is the same riboflavin with the formula C₁₇H₂₀N₄O₆, but the key difference lies in the method of production.
Production is carried out exclusively through a biotechnological method: selected industrial strains of Bacillus subtilis are cultivated in a nutrient medium, where they synthesize riboflavin in significant quantities. After fermentation, the product is purified and dried. This method is considered one of the most environmentally safe and aligns with the concept of “biosynthesis.”
In terms of properties, E101(iii) is identical to naturally derived riboflavin: it has an intense yellow color, limited water solubility, and sensitivity to light. It tolerates heat processing well and remains stable in most food matrices.
In the food industry, the E101(iii) additive is used as a natural colorant and vitamin supplement in “clean label” products, organic, and functional foods. Its use allows manufacturers to emphasize the biotechnological and natural origin of the ingredients.
For more detailed information on the properties of riboflavins, their effects on the human body, and references to scientific studies, see the material on the food additive E101.